I'll just be short about this error. New to IIS 7.5 Windows Server 2008 R2 and have issues with permissions write access on folders that the application is using?
Write Access to folders will not cut it by simply giving permissions to IUSR or IIS_IUSRS group.
If the application pool is using “ApplicationPoolIdentity” this means that the application pool has its own user account.
You need to add the App Pool use to that folder and give it access.